Jennifer Johnston

408-924-4904
珍妮花.johnston@dctdsj.com
DH 535

约翰斯顿实验室

• Postdoc, Stanford University
• Ph.D., Emory University
• M.S., Southern Illinois University

• 杂志137年
• 杂志178年

The Johnston lab is pursuing the utilization of designer nucleases to cure genetic blood disorders.  Nucleases, such as CRISPRs or TALENS, can be designed to produce a cut at a specific location in the genome.  这些 DNA breaks are then repaired by 一个 of two endogenous cellular pathways: 1) nonhomolgous enjoining or 2) homologous recombination.  If a nuclease is delivered to a cell in combination with a donor DNA template, the endogenous homologous recombination machinery can repair the break with the donor DNA.  In this way, the donor DNA can be designed to facilitate the incorporation of a gene or genes of interest into a specific location in the genome.  Therefore, blood disorders that are due to a nonfunctional gene can be corrected with a nuclease and a donor DNA template.  Due to the renewal properties of hematopoietic stem cells (HSC), the Johnston lab is currently interested in editing the genome of HSCs as a means to provide a lifelong cure to patients with Hemophilia A.

• 林根,C.B.丹宁，G.门里斯，K.Gangadharan, B.约翰斯顿，J。.M., Kerstann K.麦卡锡，D.斯宾塞，H.T. (2009) Directed 工程 of a High-Expression Chimeric Transgene as a Strategy for 其他基因apy of Hemophilia A. 摩尔其他. 17:1145-1154.
• 约翰斯顿J.M.杜林C.B.丹宁·G.斯宾塞，H.T. (2013) Generation of an optimization of a lentiviral vector encoding a high expression FVIII transgene for the gene therapy of hemophilia A. 其他基因. 20:607-615
• 约翰斯顿J.M.丹宁，G.Moot, R.怀特海德，D.希尔兹，J.勒杜，J.M.林根, C.B.斯宾塞，H.T. (2014) High-throughput screening identifies compounds that enhance lentiviral transduction. 其他基因. 21:1008-1020.